Chloroquine cell culture

Discussion in 'Without A Doctor Prescription' started by dimets, 24-Feb-2020.

  1. pash New Member

    Chloroquine cell culture


    No effective prophylactic or post-exposure therapy is currently available. We report, however, that chloroquine has strong antiviral effects on SARS-Co V infection of primate cells.

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    Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. The combination of chloroquine with zinc enhanced chloroquine’s cytotoxicity and induced apoptosis in A2780 cells. Thus chloroquine is a zinc ionophore, a property that may contribute to chloroquine’s anticancer activity. Chloroquine inhibits the intracellular multiplication of Legionella pneumophila by limiting the availability of iron. Similar to bafilomycin, the former malaria drug chloroquine CQ is now widely used as an inhibitor of autophagy in both cell culture and in vivo. Chloroquine has a long history of human use and is currently being tested as a sensitizing agent for certain cancers, making understanding its mechanisms of action both topical and important 7, 8, 9.

    In addition to the well-known functions of chloroquine such as elevations of endosomal p H, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage.

    Chloroquine cell culture

    Chloroquine-induced autophagic vacuole accumulation and cell., Chloroquine & Hydroxychloroquine supporting chemo effectiveness and.

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  3. I am using Chloroquine and its effect on autophagy and I want to treat my cell culture with a dose close to the exposition with CQ treatment. I first thought that chloroquine-treated cells.

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    The researchers concluded that “chloroquine is effective in preventing the spread of SARS CoV in cell culture.” “Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection,” they added. Cell suspensions were prepared by accutase Innovative Cell Technologies, San Diego, California treatment and plated onto uncoated 48-well plates at a density of 20 000 per well. Cultures were then incubated overnight before being used in experiments. During drug treatment, cell culture medium was switched to DMEM/F12 without FBS. I have tried daily change with medium with new chloroquine for thje autophagy studies and keep the same culture medium and chloroquine for 24-72 hours so far I have not seen difference in the results.

     
  4. abugilda33 User

    Background: The American Academy of Ophthalmology recommendations on screening for chloroquine (CQ) and hydroxychloroquine (HCQ) retinopathy are revised in light of new information about the prevalence of toxicity, risk factors, fundus distribution, and effectiveness of screening tools. PLAQUENIL HYDROXYCHLOROQUINE SULFATE TABLETS, USP DESCRIPTION Hydroxychloroquine-Induced Toxic Myopathy Causing Respiratory. Hydroxychloroquine Side Effects Common,
     
  5. Anatoliy User

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